| 9:00 - 9:15 am |
Introduction/ Schedule
for Day 1 & 2 |
|
| |
Day One |
|
| 9:15 - 11:15 |
|
Introduction to Cell Molecular Biology |
| |
|
- Cell Structure
- Plasma Membrane
- Biologically Important Organic Molecules
- Biogeochemical Cycles and Bioremediation
|
| 11:15 - 11:30 |
Break |
|
| 11:30 - 12:30 pm |
|
Foundation of Biotechnology and Genetic Engineering |
| |
|
- Origin and development of biotechnology
- Bioscience industries
- Cell growth
- Medical and food products
- DNA and protein synthesis
- DNA analysis
- RFLP and PCR
- DNA fingerprinting
|
| 12:30 - 1:30 |
Lunch |
|
| 1:30 - 3:00 |
|
Introduction to Bioinformatics |
| |
|
- Applications
- Statistics
- Literature search
- Databases
- Annotation
- Comparison
- Homology
- Sequences primer design
- Phylogenic studies
|
| 3:00 - 3:15 |
Break |
|
| 3:15 - 5:00 |
|
Basic Chemistry and computer business
Application |
| |
|
- Introduction to Chemistry
- Biochemistry
- Molecules
- Atoms, protons, neutrons and electron
|
| |
Day Two |
|
| 9:00 - 12:00 am |
|
Biotechnology Laboratory |
| |
|
light microscopy, sterile technique, calibration/use of pH meters, calibration/use of micropipetters, use of balances, preparation of reagents/buffers, specific gravity, measurement, titration, centrifugation, Spectrophotometry uv/vis/ir, chromatography, paper/gel filtration/ion exchange, TLC/GC/HPIC/HPLC, enzyme hydrolysis - carbohydrates/lipids/proteins, distillation, crystallization. |
| 12:30 - 1:30 |
Lunch |
|
| 1:30 - 5:00 |
|
Biotechnology advanced Laboratory |
| |
|
electrophoresis - SDS PAGE/Agarose, protein purification, protein assays - Lowry/Bradford, enzyme kinetics, ELISA, western blot, cell culture/hemacytometer, DNA isolation and purification, restriction digestion, cloning of recombinant DNA, plasmid prep: mini/maxi, competent cell preparation, southern blot, fluorometry, PCR, DNA sequencing, General Skills, Math Skills, standard curves, dilutions, standard deviation, CV, Computer Skills, spreadsheets - maintaining a database, graphing, teamwork, ethics, oral presentation of data, General Skills, Laboratory microbiology techniques, media prep/plate pouring/plating/culture prep/fermentation, safety practices, familiarity with SOPs, GLPs, FDA & EPA regulations, proper documentation/laboratory records and notebook standards |
| This seminar is $995.00 for two days, breakfast and lunch will be provided. |
